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NELL2 Antibody

NELL2 Antibody

Product Specifications

Product Name Alternative

NELL2, NEL-like 2 (chicken), NRP2, NEL-like protein 2, NEL-related protein 2, neural epidermal growth factor-like 2, protein kinase C-binding protein NELL2

UniProt

Q99435

Reactivity

Human

Immunogen

The immunogen for this antibody is: DPCQADTIRNDITKT

Target

NELL2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 80kDa band in Human Brain (cortex and hippocampus) lysates after 0.5μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 94.7kDa for the mature form (aa22-866) according to NP_001138579.1 and 88.8kDa (aa22-816) according to NP_001138580.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 5μg/mL.

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_001138582.1, NP_006150.1, NP_001138579.1, NP_001138581.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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