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SARS-CoV-2 (COVID-19) Spike Antibody

SARS-CoV-2 (COVID-19) Spike Antibody

Product Specifications

Product Name Alternative

SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein

UniProt

P0DTC2

Reactivity

Virus

Immunogen

Anti-SARS-CoV-2 (COVID-19) Spike antibody (orb1239976) was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Spike protein.

Target

S

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Infectious Disease & Virology

Purification

SARS-CoV-2 (COVID-19) Spike Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: 1 μg/mL; IF: 1 μg/mL. IHC: 0.2 μg/mL; Antibody validated: Immunofluorescence and Western blot in human samples. Immunohisochemistry and immunofuorescence in COVID-19 patient samples. It will detect 4 ng of free peptide at 1 μg/mL. The immunogen for this is within the last 50 aa of the spike protein - a peptide corresponding to 20 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The Extracellular domain (ECD) is from aa 1 to 1208 (full length 1273aa) . Therefore, this antibody detects the transmembrane and cytoplasm domains at the C terminus, but does not detect the ECD (which is the region expressed in many commercially available spike proteins) . This antibody can be used for the detection of full length spike protein and spike protein in COVID-19 patient samples. All other applications and species not yet tested.

Tested Applications

ELISA, IF, IHC, WB

NCBI Accession Number

QHD43416

Host or Source

Rabbit

Preservative

SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.

Isotype

IgG

Available Sizes

Frequently Asked Questions

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