Donkey anti Chicken IgY Fc, conjugated with Agarose
Product Specifications
Host
Donkey
Conjugation
IP Gel
Type
Secondary Antibodies
Source
A donkey was immunized with purified chicken IgY. Anti-IgY Fc was isolated from immune sera using affinity chromatography and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.
Purification Method
Egg-yolk derived IgY Affinity Purified
Assay Principle
The specific activity of the anti-IgY-agarose was determined by applying 2.7 mg IgY in 24 mL PBS (0.1 mg/mL) to 1.0 mL bed volume of agarose. Two mL aliquots were collected. The total amount of IgY that bound to the column was calculated. For this lot, 1.3 mg IgY bound per mL agarose.
Form
Gel; 2,0 mL bed volume, Phosphate buffered saline‚ pH 7,2 with 0,075% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
Storage Conditions
Product should be stored at 4-8ºC; DO NOT FREEZE
CAS Number
9007-83-4
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