Rabbit Chicken IgY (H&L) Antibody (Alkaline Phosphatase)

Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgY coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgY and Chicken Serum.

Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 mg of Chicken IgY in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product. (Optimal dilution should be determined by user) .

0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0; 0.1% (w/v) Sodium Azide; 10 mg/mL Bovine Serum Albumin (rAlbumin)