Spectinomycin ELISA Kit

Spectinomycin is an aminocyclitol drug produced by the soil microorganism Streptomyces spectabilis. The drug is effective against gram-positive and gram-negative bacteria and is used to treat gonorrheal infections. Spectinomycin works by binding to the 30S ribosomal subunit and thus inhibiting bacterial protein synthesis. The drug is used in animals not bred for human consumption to treat bacterial respiratory and enteric infections. BioVision’s Spectinomycin ELISA kit is used to quantitatively measure Spectinomycin in tissue and milk samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Spectinomycin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Spectinomycin in the samples.