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Anti-MyoD (Phospho-Ser200) Antibody

Rabbit Polyclonal Antibody specific to MyoD (Phospho-Ser200)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-MyoD (Phospho-Ser200) Rabbit Polyclonal Antibody

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of Serine 200 (A-S-S(p)-P-R) derived from human MyoD and conjugated to KLH.

Target

MyoD (Phospho-Ser200)

Clonality

Polyclonal

Type

Antibody

Applications

WB

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~40kDa is detected. <br><br>These are recommended working dilutions. <br><br>End users should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human, mouse, and rat MyoD only when phosphorylated at serine 200.Accession no.: P15172, NP_002469.2

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

MyoD encodes a nuclear protein that is a member of the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest which is required for myogenic initiation. MyoD is also involved in muscle regeneration. It activates its own transcription which may stabilize the commitment to myogenesis.

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