Fluorescent Human Claudin-6 / CLDN6 Full Length Protein, Flag, His, GFP Tag (Nanodisc)
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space.
Product Specifications
Background
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space.
Specifications
This protein carries a flag tag at the N-terminus, followed by a polyhistidine tag, and a GFP tag at the C-terminus. The protein has a calculated MW of 54.0 kDa. The protein migrates as 22 kDa, 25 kDa, 51-52 kDa and 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P56747
Expression Region
Ala 2 - Val 220
Host
HEK293
Target
Claudin-6
Conjugation
GFP Fusion
Tag
N-Flag & N-10xHis & C-GFP
Source
Human
Stability
The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.
Endotoxin
1.0 EU per μg
Purity
80%
Bioactivity
Immobilized Fluorescent Human Claudin-6 Protein, Flag,His,GFP Tag (Cat. No. CL6-HF2M9) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Claudin 6 antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
Format
Liquid
Buffer
20 mM HEPES, 150 mM NaCl, pH7.5
Additives
Trehalose
Molecular Weight
54.0 kDa
Additionnal Information
Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments
Shipping Conditions
Dry ice
Storage Conditions
-70°C
Package Size
20ug*1
Host or Source
HEK293
Species
Human
Protein ID
P56747
Preservative
Trehalose
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