CheKine™ Micro Total Sulfhydryl Group Content Detection Kit

The thiol groups in living organisms mainly include glutathione thiol and protein thiol. The former not only repairs proteins damaged by oxidation, but also participates in the clearance of reactive oxygen species, while the latter plays an important role in maintaining protein conformation. By measuring the total sulfhydryl group content and glutathione content, protein thiol content can be indirectly determined. The thiol group reacts with 5, 5'- dithio bis nitrobenzoic acid (DTNB) to form a yellow compound with a maximum absorption peak at 412 nm.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Storage at ４°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.