5M Betaine Solution (PCR Grade)

<strong>5M Betaine Solution PCR Grade</strong>_x000D_ <strong>Catalog number:</strong> B2010141_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Volume/Weight:</strong> 5 x 2 mL_x000D_ <strong>pH:</strong> na_x000D_ <strong>Supplied as:</strong> Ready-to-use solution_x000D_ <strong>Appearance:</strong> Clear solution_x000D_ <strong>Applications:</strong> stock solution of betaine (also known as trimethylglycine or N,N,N triethylammonium acetate) which is an analog of glycine with three methyl groups. Betaine is useful in PCR applications because it has an effect on the melting temperature difference between GC and AT base pairs in DNA._x000D_ <strong>Storage:</strong> 2-8°C_x000D_ <strong>Keywords:</strong> Betaine solution, Betaine PCR_x000D_ <strong>Grade:</strong> Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Day CR, Kempson SA. Betaine chemistry, roles, and potential use in liver_x000D_ disease. Biochim Biophys Acta. 2016 Jun;1860(6):1098-106._x000D_ _x000D_ 2: Figueroa-Soto CG, Valenzuela-Soto EM. Glycine betaine rather than acting only_x000D_ as an osmolyte also plays a role as regulator in cellular metabolism. Biochimie._x000D_ 2018 Apr;147:89-97._x000D_ _x000D_ 3: Zou H, Chen N, Shi M, Xian M, Song Y, Liu J. The metabolism and_x000D_ biotechnological application of betaine in microorganism. Appl Microbiol_x000D_ Biotechnol. 2016 May;100(9):3865-76._x000D_ _x000D_ 4: Servillo L, D'Onofrio N, Giovane A, Casale R, Cautela D, Ferrari G, Castaldo_x000D_ D, Balestrieri ML. The betaine profile of cereal flours unveils new and uncommon_x000D_ betaines. Food Chem. 2018 Jan 15;239:234-241._x000D_ _x000D_ 5: Koistinen VM, Kärkkäinen O, Borewicz K, Zarei I, Jokkala J, Micard V, Rosa-_x000D_ Sibakov N, Auriola S, Aura AM, Smidt H, Hanhineva K. Contribution of gut_x000D_ microbiota to metabolism of dietary glycine betaine in mice and in vitro colonic_x000D_ fermentation. Microbiome. 2019 Jul 10;7(1):103._x000D_ _x000D_ 6: Su Y, Guo QQ, Wang S, Zhang X, Wang J. Effects of betaine supplementation on_x000D_ L-threonine fed-batch fermentation by Escherichia coli. Bioprocess Biosyst Eng._x000D_ 2018 Oct;41(10):1509-1518._x000D_ _x000D_ 7: Luo GC, Yi TT, Jiang B, Guo XL, Zhang GY. Betaine-assisted recombinase_x000D_ polymerase assay with enhanced specificity. Anal Biochem. 2019 Jun 15;575:36-39._x000D_ _x000D_ 8: Cabezón FA, Stewart KR, Schinckel AP, Barnes W, Boyd RD, Wilcock P, Woodliff_x000D_ J. Effect of natural betaine on estimates of semen quality in mature AI boars_x000D_ during summer heat stress. Anim Reprod Sci. 2016 Jul;170:25-37._x000D_ _x000D_ 9: Lever M, Slow S. The clinical significance of betaine, an osmolyte with a key_x000D_ role in methyl group metabolism. Clin Biochem. 2010 Jun;43(9):732-44._x000D_ _x000D_ 10: Bhojane PP, Duff MR Jr, Bafna K, Rimmer GP, Agarwal PK, Howell EE. Aspects_x000D_ of Weak Interactions between Folate and Glycine Betaine. Biochemistry. 2016 Nov_x000D_ 15;55(45):6282-6294._x000D_ _x000D_ 11: López-Rojas R, Fernández-Cuenca F, Serrano-Rocha L, Pascual Á. In vitro_x000D_ activity of a polyhexanide-betaine solution against high-risk clones of_x000D_ multidrug-resistant nosocomial pathogens. <a href="https://pubmed.ncbi.nlm.nih.gov/27004429/">Enferm Infecc Microbiol Clin</a>. 2017_x000D_ Jan;35(1):12-19._x000D_ _x000D_ <strong>Products Related to 5M Betaine Solution PCR Grade: </strong> <a>Biochemical Buffers and Solutions</a>