Double stranded RNA control Poly(I:C)

The low molecular weight Poly(I:C) dsRNA sequence is intended to be used as a positive control in double-stranded (ds)RNA detection. The sequence does not contain any viral or potentially transforming elements._x000D_ _x000D_ Positive control for dsRNA detection by anti dsRNA monoclonal J2, J5, K1 and K2 antibodies._x000D_ _x000D_ Caution: The Poly(I:C) dsRNA positive control, to be combined with our monoclonal anti dsRNA antibodies J2, J5, K1 and K2 in both the direct AN-ELISA and sandwich ELISA, is not intended to be used as a quantitative standard for other dsRNA preparations. The anti dsRNA antibodies may exhibit a different degree of reactivity with different dsRNAs obtained from natural sources. It is therefore only intended to be used as a positive control to see if the ELISA has been executed correctly and that the test shows a linear relationship between the amount of dsRNA and the read out, for example the OD450, in the 4-parameter analysis. It cannot be used to determine the concentration of a different type of dsRNA. The only proper standard for each specific application is the purified dsRNA under investigation.

Double stranded RNA control Poly(I:C) is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels. We sµggest to use it as a homogenous, well-characterized positive control for different dsRNA detection methods, e.g. for ELISA and dot-blots._x000D_ _x000D_ The optimum working dilution of the Poly(I:C) dsRNA control for any specific application should be established by titration.

Each vial contains 50 μl of a 1 mg/mL Poy(I:C) solution in RNAse/DNAse-free STE Buffer (0,1 M NaCl, 1 mM EDTA, 50 mM Tris-HCl, pH 7,0). Low molecµLar weight polyinosine-polycytidylic acid (i.e. poly(I:C)) is a synthetic analog of double-stranded RNA (dsRNA) and is comprised of short strands of inosine poly(I) homopolymer annealed to strands of cytidine poly(C) homopolymer; The average size of Poly(I:C) LMW is 0,2 kb to 1 kb.