5-IAF

5-IAF (5-Iodoacetamidofluorescein) is an idoacetamide derivate of fluoresceine. 5-IAF can be used as fluorescent probe that labels proteins and other molecules having free thiols (cysteine side chains) [1][2].

Product Specifications

CAS Number

[63368-54-7]

Product Name Alternative

5-Iodoacetamidofluorescein

UNSPSC

12171500

Hazard Statement

H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Applications

Cancer-programmed cell death

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/5-IAF.html

Purity

95.10

Solubility

DMSO : 50 mg/mL (ultrasonic)

Smiles

O=C(NC1=CC2=C(C3(C4=C(OC5=C3C=CC(O)=C5)C=C(O)C=C4)OC2=O)C=C1)CI

Molecular Formula

C22H14INO6

Molecular Weight

515.25

Precautions

H315, H319, H335

References & Citations

[1]Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013; 8 (7) : e70374.|[2]Yan Wang, et al. Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2009 Apr 17;1216 (16) :3533-7.