Recombinant Human Interleukin-23
Product Specifications
Purification
> 95 % by SDS-PAGE analyses.
Endotoxin
Less than 1.0 EU/ug of rHuIL-23 as determined by LAL method.
Bioactivity
Measured by its ability to induce IL-17 secretion by mouse splenocytes. The ED50 for this effect is 0.05-0.3 ng/ml.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculated Molecular Weight
Apparent molecular mass of 65 kDa, 43-45 kDa and 20 kDa in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from 0.2 μm filtered concentrated sterile solution in 20 mM MES, pH 6.0, 500 mM NaCl and 1 mM EDTA.
Host or Source
Spodoptera frugiperda, Sf 21 (baculovirus)
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