Recombinant Human CCL4

Recombinant human CCL4

Product Specifications

CAS Number

9000-83-3

Product Name Alternative

rHuCCL4, MIP-1beta

Gene Name

CCL4

UniProt

P13236

Cellular Locus

Secreted.

Reactivity

Human

Target Antigen

C-C motif chemokine 4

Target

CCL4

Type

Protein

Applications

Migration assay

Dilution

Centrifuge tube prior to resuspending. Recommended at 100ug/ml in sterile distilled water.

Form

Lyophilized

Function

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. RPubMed:10540332, PubMed:12070155, PubMed:8525373}.

Additionnal Information

Endotoxin Level: <0.01 EU per 1ug of protein by LAL method.Migration Assay in cells expressing recombinant CCR5.

Storage Conditions

12 months from date of receipt, -20°C to -70°C, as supplied. 1 month,- 20°C to -70°C, under sterile conditions after reconstitution. Best if used immediately after reconstitution. Avoid multiple freeze-thaw cycles.

Specificity

CCL4

Target Background

CCL4, also known as Macrophage Inflammatory Protein-1beta (MIP-1b) is a small cytokine (~9kDa) that belongs to the CC chemokine family. CCL4 binds to CCR1, CCR2 isoform B, and CCR5. CCL4 is one of the major HIV-suppressive factors produced by CD8+ T-cells; recombinant MIP-1b induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form of MIP-1b (aa 3-69) retains the abilities to induce down-modulation of surface expression of CCR5 and to inhibit CCR5-mediated entry of HIV-1 into T-cells.