Anti-Akt (Phospho-Ser473) Antibody

Rabbit Polyclonal Antibody specific to Akt (Phospho-Ser473)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Akt (Phospho-Ser473) Rabbit Polyclonal Antibody

UniProt

P31749

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation sites of Serine 473 (Q-F-S(p)-Y-S) derived from human Akt and conjugated to KLH.

Target

Akt (Phospho-Ser473)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~60kDa is detected. Immunohistochemistry: use at dilution of 1:50-1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human, mouse, and rat Akt only when phosphorylated at serine 473.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Akt, also known as Akt1 and Protein kinase B (PKB), is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration. Akt1 is also able to induce protein synthesis pathways and is, therefore, a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. Since it can block apoptosis, and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer.