Horseradish Peroxidase Biotinylated

<strong>Horseradish Peroxidase Biotinylated</strong>_x000D_ <table width="270">_x000D_ <tbody>_x000D_ <tr>_x000D_ <td>Catalog number:</td>_x000D_ <td>P2010010</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Lot number:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Expiration date:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Package Size:</td>_x000D_ <td>1 mg</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Specific Activity:</td>_x000D_ <td>350 U/mg</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Unit Definition:</td>_x000D_ <td>Amount of biotinylated HRP needed to produce 1 μmole of purpurogallin from pyrogallol in 20 seconds at 20°C in reaction buffer: 8 mM hydrogen peroxide, 14 mM potassium phosphate, 0.5% pyrogallol, pH 6.0.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Biotin Incorporation:</td>_x000D_ <td>1.3 moles biotin/mole HRP (HABA assay)</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Appearance:</td>_x000D_ <td>Lyophilized powder</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Storage:</td>_x000D_ <td>-20°C or lower. Avoid repeated freeze-thaw.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Purity:</td>_x000D_ <td>&gt;95%.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Recommended Buffer:</td>_x000D_ <td>10 mM citrate, pH 6.0</td>_x000D_ </tr>_x000D_ </tbody>_x000D_ </table>_x000D_ &nbsp;_x000D_ _x000D_ <strong>References Related to Horseradish Peroxidase Biotinylated_x000D_ </strong>_x000D_ <ul>_x000D_ <li>False biochemical diagnosis of hyperthyroidism in streptavidin-biotin-based immunoassays: the problem of biotin intake and related interferences. <span class="labs-docsum-authors full-authors">Piketty ML, Polak M, Flechtner I, Gonzales-Briceño L, Souberbielle JC.</span> <span class="labs-docsum-journal-citation full-journal-citation">Clin Chem Lab Med. 2017 May 1;55(6):780-788. </span></li>_x000D_ <li>Characterization of the scope and magnitude of biotin interference in susceptible Roche Elecsys competitive and sandwich immunoassays.<span class="labs-docsum-authors full-authors"> Trambas C, Lu Z, Yen T, Sikaris K.</span> <span class="labs-docsum-journal-citation full-journal-citation">Ann Clin Biochem. 2018 Mar;55(2):205-215.</span></li>_x000D_ <li>Depletion of biotin using streptavidin-coated microparticles: a validated solution to the problem of biotin interference in streptavidin-biotin immunoassays.<span class="labs-docsum-authors full-authors"> Trambas C, Lu Z, Yen T, Sikaris K.</span> <span class="labs-docsum-journal-citation full-journal-citation">Ann Clin Biochem. 2018 Mar;55(2):216-226.</span></li>_x000D_ <li>Current good manufacturing practices-compliant manufacture and measurement of biotin-labeled red blood cells.<span class="labs-docsum-authors full-authors"> Donnenberg AD, Kanias T, Triulzi DJ, Dennis CJ, Moore LR, Meyer EM, Sinchar D, Kiss JE, Normolle DP, Gladwin MT.</span> <span class="labs-docsum-journal-citation full-journal-citation">Cytotherapy. 2019 Jul;21(7):793-800.</span></li>_x000D_ <li>Characterization of biotin interference in 21 Vitros 5600 immunoassays and risk mitigation for patient safety at a large academic medical center.<span class="labs-docsum-authors full-authors"> Stieglitz HM, Korpi-Steiner N.</span> <span class="labs-docsum-journal-citation full-journal-citation">Clin Biochem. 2020 Jan;75:53-61. </span></li>_x000D_ <li>Availability of NHS-biotin labeling to identify free protein lysine revealed by experiment and MD simulation.<span class="labs-docsum-authors full-authors"> Muraoka A, Matsuura Y, Naitow H, Ihara M, Kunishima N.</span> <span class="labs-docsum-journal-citation full-journal-citation">Anal Biochem. 2018 Sep 15;557:46-58.</span></li>_x000D_ <li>Biotin accounts for less than half of all biotin and biotin metabolites in the cerebrospinal fluid of children.<span class="labs-docsum-authors full-authors"> Bogusiewicz A, Stratton SL, Ellison DA, Mock DM.</span> <span class="labs-docsum-journal-citation full-journal-citation">Am J Clin Nutr. 2008 Nov;88(5):1291-6. </span></li>_x000D_ <li>Endogenous biotin in rat brain: implications for false-positive results with avidin-biotin and streptavidin-biotin techniques.<span class="labs-docsum-authors full-authors"> McKay BE, Molineux ML, Turner RW.</span> <span class="labs-docsum-journal-citation full-journal-citation"><a href="https://pubmed.ncbi.nlm.nih.gov/18287654/">Methods Mol Biol.</a> 2008;418:111-28. </span></li>_x000D_ </ul>