Sarafloxacin ELISA Kit

Sarafloxacin is a veterinary fluoroquinolone antimicrobial agent that inhibits bacterial DNA gyrase. It has been used for the control of bacterial infections in poultry caused by either E. coli or Salmonella species. Norfloxacin ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Sarafloxacin. During the reaction, Sarafloxacin in the samples or standard competes with Sarafloxacin coated on the plate for binding to the anti-Sarafloxacin antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Sarafloxacin. The concentration of Sarafloxacin in the samples can be calculated by comparing the OD of the samples to the standard curve.