Anti-Neuroligin-1 Antibody

Mouse Monoclonal Antibody specific to Neuroligin-1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Neuroligin I

Gene Name

Nlgn1

NCBI Gene ID

UniProt

Q62765

Cellular Locus

Cell membrane; Single-pass type I membrane protein. Cell junction, synapse, postsynaptic density, Cell junction, synapse, synaptic cleft, Cell junction, synapse, synaptic cell membrane

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 718-843 (cytoplasmic C-terminus) of rat Neuroligin- 1, accession no. Q62765). This sequence is 99

Target Antigen

Neuroligin-1

Target

Neuroligin-1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Cell surface protein involved in cell-cell-interactions via its interactions with neurexin family members. Plays a role in synapse function and synaptic signal transmission, and probably mediates its effects by recruiting and clustering other synaptic proteins. May promote the initial formation of synapses, but is not essential for this. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Required to maintain wakefulness quality and normal synchrony of cerebral cortex activity during wakefulness and sleep (By similarity). The protein is involved in nervous system development. {UniProtKB:Q99K10, PubMed:15620359, PubMed:9325340}.

Additionnal Information

Immunoblotting: use at 1-5ug/mL. A band of ~120kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This product is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat Neuroligin-1. It does not cross- react with other Neuroligins.

Formulation

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

Buffer pH

pH 7.4

Target Background

Neuroligin-1 is a neuronal cell surface protein and member of the type-B carboxylesterase/lipase family. It is a necessary component in the maturation of excitatory synapses, it participates in regulation of synaptic plasticity, and it is important in the development of long- term memory in adult mammalian amygdala. It's participation in cell-cell interactions may occur through assembly of intracellular junctions by binding of beta-neurexins. In nervous system tissue, Neuroligin-1 is observed primarily in neurons and in the spinal cord.