Human IL15RA & IL15 Protein, Fc Tag (N120D)

Interleukin-15 is a member of the four α helix bundle family of cytokines. It plays a multifaceted role in the development and control of the immune system. The IL-15R consists of three polypeptides, the type-specific IL-15Rα, the IL-2/IL-15Rβ, and the common γ-chain (γC), which is shared by multiple cytokine receptors. IL-15 could enhance the effect of chemotherapeutic agents and ACT on transplanted tumors, but had little or no effect when administered alone. The result of research shows that the biological activity of IL-15 could be increased ∼50-fold by administering preformed complexes of IL-15 and its soluble receptor, IL-15Rα.

Product Specifications

Background

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 48.0 kDa. The protein migrates as 53-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q13261-1 & P40933-1 (N120D)

Expression Region

Ile 31 - Arg 95 (IL15RA) & Asn 49 - Ser 162 (IL15)

Host

HEK293

Target

IL15RA & IL15

Conjugation

Unconjugated

Tag

C-Fc

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (Cat. No. ILG-H5283) at 2 μg/mL (100 μL/well) can bind Human IL15RA & IL15 Protein, Fc Tag (N120D) (Cat. No. IL5-H5256) with a linear range of 0.1-4 ng/mL (QC tested).