Anti-Copper-Transporting ATPase1 Antibody

Mouse Monoclonal Antibody specific to Copper-Transporting ATPase1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 7.2.2.8, Copper pump 1, Menkes disease-associated protein

Gene Name

ATP7A

NCBI Gene ID

UniProt

Q04656

Cellular Locus

Golgi apparatus, trans-Golgi network membrane, Cell membrane, Melanosome membrane, Early endosome membrane, Cell projection, axon, Cell projection, dendrite, Cell junction, synapse, postsynaptic density

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide correspond- ing to aa 42-61 (cytoplasmic C-terminus) of human Copper-Transporting ATPase1 (accession no. NP_000043.3).

Target Antigen

Copper-transporting ATPase 1

Target

Copper-Transporting ATPase1

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

WB, IHC, ICC/IF, IP

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

ATP-driven copper (Cu(+)) ion pump that plays an important role in intracellular copper ion homeostasis (PubMed:10419525, PubMed:11092760, PubMed:28389643). Within a catalytic cycle, acquires Cu(+) ion from donor protein on the cytoplasmic side of the membrane and delivers it to acceptor protein on the lumenal side. The transfer of Cu(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state (PubMed:10419525, PubMed:19453293, PubMed:19917612, PubMed:31283225, PubMed:28389643). Under physiological conditions, at low cytosolic copper concentration, it is localized at the trans-Golgi network (TGN) where it transfers Cu(+) ions to cuproenzymes of the secretory pathway (PubMed:28389643, PubMed:11092760). Upon elevated cytosolic copper concentrations, it relocalizes to the plasma membrane where it is responsible for the export of excess Cu(+) ions (PubMed:10419525, PubMed:28389643). May play a dual role in neuron function and survival by regulating cooper efflux and neuronal transmission at the synapse as well as by supplying Cu(+) ions to enzymes such as PAM, TYR and SOD3 (PubMed:28389643) (By similarity). In the melanosomes of pigmented cells, provides copper cofactor to TYR to form an active TYR holoenzyme for melanin biosynthesis (By similarity). {UniProtKB:Q64430, PubMed:10419525, PubMed:11092760, PubMed:19453293, PubMed:19917612, PubMed:28389643, PubMed:31283225}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~180kDa is detected.

Storage Conditions

This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat Copper-Transporting ATPase1.Immunohistochemistry: use at 1-5ug/ml. These are recommended concentrations.Enduser should determine optimal concentrations for their

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

The copper efflux transporters ATP7A and ATP7B sequester intracellular copper into the vesicular secretory pathway for export from cells. ATP7A (also known as Copper-Transporting ATPase1) functions as a transmembrane copper-translocating P-type ATPase and plays a vital role in systemic copper absorption in the gut and copper reabsorption in the kidney. Although ATP7A is not detectable in most normal tissues, it is expressed in many common tumor types. Increased expression of ATP7A renders tumor cells resistant to cisplatin and carboplatin.