5-bromo-4-chloro-3-indolyl β-D-galactopyranoside

<strong>5-bromo-4-chloro-3-indolyl β-D-galactopyranoside</strong>_x000D_ <strong>Catalog number:</strong> B2012172_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 50 mg_x000D_ <strong>Molecular Weight or Concentration:</strong> 408.63 g/mol_x000D_ <strong>Supplied as:</strong> Powder_x000D_ <strong>Applications:</strong> molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> -20°C_x000D_ <strong>Keywords:</strong> 5-Bromo-4-chloro-3-(β-D-galactopyranosyloxy)indole, X-Gal_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Itahana K, Campisi J, Dimri GP. Methods to detect biomarkers of cellular senescence: the senescence-associated beta-galactosidase assay Methods Mol Biol. 2007;371:21-31._x000D_ 2: Gary RK, Kindell SM. Quantitative assay of senescence-associated beta-galactosidase activity in mammalian cell extracts Anal Biochem. 2005 Aug 15;343(2):329-34._x000D_ 3: Aspelund A, Antila S, Proulx ST, Karlsen TV, Karaman S, Detmar M, Wiig H, Alitalo K. A dural lymphatic vascular system that drains brain interstitial fluid and macromolecules J Exp Med. 2015 Jun 29;212(7):991-9._x000D_ 4: Green MR, Sambrook J. Screening Bacterial Colonies Using X-Gal and IPTG: α-Complementation Cold Spring Harb Protoc. 2019 Dec 2;2019(12)._x000D_ 5: Lozano-Gerona J, García-Otín ÁL. ImageJ-based semiautomatic method to analyze senescence in cell culture Anal Biochem. 2018 Feb 15;543:30-32._x000D_ 6: Rodig SJ. Detecting β-Galactosidase-Labeled Cells Cold Spring Harb Protoc. 2020 May 1;2020(5):099739._x000D_ 7: Arakawa H, Ikegami T, Maeda M, Tsuji A. Chemiluminescent enzyme immunoassay for alpha-fetoprotein using beta-D-galactosidase as label and 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside as substrate J Biolumin Chemilumin. 1993 May-Jun;8(3):135-9._x000D_ 8: Ley A, Barr S, Fredenburgh D, Taylor M, Walker N. Use of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside for the isolation of beta-galactosidase-positive bacteria from municipal water supplies Can J Microbiol. 1993 Sep;39(9):821-5._x000D_ 9: Kobayashi S, Okada M. A double staining technique using 5-bromo,4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) and immunoperoxidase in whole Drosophila embryos Biotech Histochem. 1993 Jul;68(4):237-9._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/33606204">10: Krämer MS, Feil R, Schmidt H. Analysis of Gene Expression Using lacZ Reporter Mouse Lines Methods Mol Biol. 2021;2224:29-45. </a>_x000D_ _x000D_ <strong>Products Related to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside can be found at</strong> <a href="https://moleculardepot.com/product-category/Substrates/"> Substrates</a>