5-Iodoacetamidofluorescein (5-IAF)

<strong>5-Iodoacetamidofluorescein (5-IAF)</strong>_x000D_ <strong>Catalog number:</strong> B2011353_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 5 mg_x000D_ <strong>Molecular Weight or Concentration:</strong> 515.3 g/mol_x000D_ <strong>Supplied as:</strong> Lyophilized_x000D_ <strong>Applications:</strong> molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> -20 °C_x000D_ <strong>Keywords:</strong> 5-IAF [5-Iodoacetamidofluorescein]_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Sotgia S, Pisanu E, Pintus G, Erre GL, Pinna GA, Deiana L, Carru C, Zinellu A. Plasma L-ergothioneine measurement by high-performance liquid chromatography and capillary electrophoresis after a pre-column derivatization with 5-iodoacetamidofluorescein (5-IAF) and fluorescence detection PLoS One. 2013 Jul 29;8(7):e70374._x000D_ 2: Steinberg M, Karlish SJ. Studies on conformational changes in Na,K-ATPase labeled with 5-iodoacetamidofluorescein J Biol Chem. 1989 Feb 15;264(5):2726-34._x000D_ 3: Hellen EH, Pratap PR. Nucleotide binding to IAF-labelled Na+/K(+)-ATPase measured by steady state fluorescence quenching by TNP-ADP Biophys Chem. 1997 Dec 1;69(2-3):107-24._x000D_ 4: Schmidt M, Tschödrich-Rotter M, Peters R, Krohne G. Properties of fluorescently labeled Xenopus lamin A in vivo Eur J Cell Biol. 1994 Oct;65(1):70-81._x000D_ 5: Alhathal N, San Gabriel M, Zini A. Beneficial effects of microsurgical varicocoelectomy on sperm maturation, DNA fragmentation, and nuclear sulfhydryl groups: a prospective trial Andrology. 2016 Nov;4(6):1204-1208._x000D_ 6: Liu P, Huang J, Zhong L. Role and mechanism of homocysteine in affecting hepatic protein-tyrosine phosphatase 1B Biochim Biophys Acta Gen Subj. 2019 May;1863(5):941-949._x000D_ 7: Muddukrishna SN, Chen A, Qi P, Smolenski MA. Quantitation of reduced disulfide groups in monoclonal antibodies using 5-iodoacetamidofluorescein: a novel size exclusion-HPLC technique Appl Radiat Isot. 1995 Oct;46(10):1015-26._x000D_ 8: Kapakos JG, Steinberg M. 5-Iodoacetamidofluorescein-labeled (Na,K)-ATPase. Steady-state fluorescence during turnover J Biol Chem. 1986 Feb 15;261(5):2090-5._x000D_ 9: Musenga A, Mandrioli R, Bonifazi P, Kenndler E, Pompei A, Raggi MA. Sensitive and selective determination of glutathione in probiotic bacteria by capillary electrophoresis-laser induced fluorescence Anal Bioanal Chem. 2007 Feb;387(3):917-24._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/3850090">10: Bock PE, Shore JD, Tans G, Griffin JH. Protein-protein interactions in contact activation of blood coagulation. Binding of high molecular weight kininogen and the 5-(iodoacetamido) fluorescein-labeled kininogen light chain to prekallikrein, kallikrein, and the separated kallikrein heavy and light chains J Biol Chem. 1985 Oct 15;260(23):12434-43.</a>_x000D_ _x000D_ <strong>Products Related to 5-Iodoacetamidofluorescein (5-IAF) can be found at</strong> <a href="https://moleculardepot.com/product-category/Chemicals/"> Chemicals</a>