HotStart Tth DNA Polymerase

HotStart Tth DNA Polymerase contains high-performance Tth DNA Polymerase bound to a proprietary antibody, and it is supplied in an inactive state and has no polymerase activity at ambient temperatures. This prevents extension of nonspecifically annealed primers and primer dimers formed at low temperatures during PCR setup and the initial PCR cycle. The antibody is denatured in the initial DNA denaturation step of the PCR reaction, then the Tth DNA Polymerase activity is restored. It is a thermostable DNA polymerase with intrinsic reverse transcriptase (RT) activity. The enzyme is a highly processive 5´→3´ DNA polymerase and lacks 3´→5´ exonuclease activity (proof reading). It possesses a very efficient intrinsic reverse transcriptase (RT) activity in the presence of manganese ions and unlike other RT enzymes.