Cynomolgus / Rhesus macaque PTK7 / CCK4 Protein, His Tag (MALS verified)

Cynomolgus / Rhesus macaque PTK7, His Tag (PT7-C52H3) is expressed from human 293 cells (HEK293) . It contains AA Ala 39 - Thr 712 (Accession # A0A2K5VNI5-1) . In the region Ala 39 - Thr 712, the AA sequence of Cynomolgus and Rhesus macaque PTK7 are homologus.

Product Specifications

Background

PTK7, highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 76.5 kDa. The protein migrates as 100-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

A0A2K5VNI5-1

Expression Region

Ala 39 - Thr 712

Host

HEK293

Target

PTK7

Conjugation

Unconjugated

Tag

C-10xHis

Source

Cynomolgus / Rhesus macaque

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Cynomolgus / Rhesus macaque PTK7, His Tag (Cat. No. PT7-C52H3) at 1 μg/mL (100 μL/well) can bind Cofetuzumab Pelidotin with a linear range of 0.06-2 ng/mL (Routinely tested).