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Anti-Bcl-xL (Phospho-Ser62) Antibody

Rabbit Polyclonal Antibody specific to Bcl-xL (Phospho-Ser62)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Bcl-xL (Phospho-Ser62) Rabbit Polyclonal Antibody

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of Serine 62 derived from human Bcl-xL and conjugated to KLH.

Target

Bcl-xL (Phospho-Ser62)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC, IF

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A ban of ~30kDa is detected. <br><br>Immunohistochemistry: use at dilution of 1:50-1:100. <br><br>Immunofluorescence: use at dilution of 1:100-1:200. <br><br>These are recommended working dilutions. <br><br>End user should determine optimal dilutions for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human, mouse, and rat Bcl-xL only when phosphorylated at serine 62.Accession no.: NP_612815.1

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

B-cell lymphoma-extra large (Bcl-xl, or BCL2-like 1 isoform 1) is a transmembrane protein in the mitochondria. It is a member of the Bcl-2 family of proteins and acts as a pro-survival protein by preventing the release of mitochondrial cytochrome c, which would lead to caspase activation. Relative amounts of pro- and anti-survival Bcl-2 family proteins define whether a cell will undergo cell death: if more Bcl-xL is present, then pores are non-permeable to pro-apoptotic molecules and the cell survives.

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