Cap 1 Capping System

The Vaccinia Cap System uses the Vaccinia Cap Enzyme and related components to add the 7-methylguanosine cap structure (m7Gppp, Cap0) to the 5´ terminals of RNA. In eukaryotes, this structure is closely related to mRNA stabilization, transport, and translation. The enzymatic capping method is a simple and effective method, and the Cap structure is completely consistent with the natural Cap 0 structure, which can significantly improve the stability and translation ability of RNA used for in vitro transcription, transfection, and microinjection. This enzyme has two subunits (D1 and D12) that consists of the functions of RNA triphosphatase, guanosine methyltransferase, and guanine methyltransferase, both of which are necessary for the addition of a complete Cap 0 structure. The capped RNA product constructed by Vaccinia Cap Enzyme has a "cap 0" structure. By using both mRNA Cap 2' -O-methyltransferase and Vaccinia Cap Enzyme in the capping reaction, Cap 0-RNA can be converted into a "cap 1" structure. mRNA Cap 2' -O-methyltransferase produces cap 1-RNA from Cap 0-RNA by transferring methyl from the donor molecule SAM to the 2'-O position of the 5 'end of Cap 0-RNA next to the first nucleotide of the cap structure. The system can be used for the capping reaction of IVT RNA, the capping reaction can be completed within 1h, the efficiency is close to 100%, and the capping direction is right. Novoprotein T7 High Yield RNA Transcription kit (Cat. No.: E131) is recommended for IVT.