Hieff UNICON™ Hotstart E-Taq DNA Polymerase, 20 U/μL

Hieff UNICON™ Hotstart E-Taq DNA Polymerase features an enhanced hot start Taq DNA polymerase, incorporating dual blocking antibodies. Both the 5'→3' polymerase and 5'→3' exonuclease activities of Taq DNA polymerase are effectively inhibited. A 30-second heating step at the pre-denaturation temperature serves to completely deactivate the antibodies, restoring DNA polymerase and exonuclease activities. This dual blocking capability not only safeguards against nonspecific amplification due to mismatch or primer dimer but also proficiently restrains the reduction in fluorescence signal caused by probe degradation. This ensures enhanced stability of the in vitro detection reagent during transportation or room temperature usage.