Human OSCAR / PIgR-3 Protein, His Tag (MALS verified)

OSCAR (Osteoclast-associated immunoglobulin-like receptor), also known as PIgR-3 (polymeric immunoglobulin-like receptor), is a type I transmembrane protein of the leukocyte receptor complex (LRC) family. OSCAR is specifically expressed by pre-osteoclasts and signals via FcR gamma. OSCAR co-stimulates one of the major FcR gamma -associated pathways required for osteoclastogenesis in vivo. Collagen type I and collagen type II serve as ligands for OSCAR. OSCAR binds to specific motifs within fibrillary collagens in the extracellular matrix (ECM) . OSCAR promoted osteoclastogenesis in vivo, and its binding to the collagen motif led to signaling that increased numbers of osteoclasts in culture. OSCAR contains two immunoglobulin-like domains, D1 and D2. Direct binding assays on OSCAR molecules showed that D2, but not D1, is critical for collagen binding. OSCAR may contribute to the pathogenesis and severity of a number of diseases including osteoporosis, atherosclerosis, chronic obstructive pulmonary disease and rheumatoid arthritis.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.0 kDa. The protein migrates as 33-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q8IYS5-2

Expression Region

Asp 19 - Asn 229

Host

HEK293

Target

OSCAR

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Native Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human OSCAR, His Tag (Cat. No. OSR-H52H3) with a linear range of 0.039-1.25 μg/mL (QC tested).