Rhesus macaque Siglec-15 / CD33L3 Protein, Fc Tag

Rhesus macaque Siglec-15 Protein, Fc Tag (SG5-C5253) is expressed from human 293 cells (HEK293) . It contains AA Phe 20 - Thr 263 (Accession # F7ETM4) .

Product Specifications

Background

Siglec-15 is a DAP12-associated immunoreceptor, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Siglecs are cell surface proteins that bind sialic acid. They are found primarily on the surface of immune cells and are a subset of the I-type lectins. Siglec-15 consisting of immunoglobulin (Ig) -like domains, transmembrane domain and a short cytoplasmic tail. Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.

Specifications

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52.7 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

F7ETM4

Expression Region

Phe 20 - Thr 263

Host

HEK293

Target

Siglec-15

Conjugation

Unconjugated

Tag

C-Fc

Source

Rhesus macaque

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Rhesus macaque Siglec-15, Fc Tag (Cat. No. SG5-C5253) at 5 μg/mL (100 μL/well) can bind Mouse Human chimeric MAb (5G12, Human IgG1) with a linear range of 0.4-6 ng/mL (QC tested).