Anti-Influenza A Virus H1N1 Antibody

This antibody recognizes H1N1 HA (~75kDa), NA (~55kDa), M (~26kDa) and NP (~26-27kDa). M and NP often co- migrate as a single band in SDS-PAGE. It also reacts with A/New Caledonia/20/99, and A/Brisbane/10/2007. Other isolates of H1N1 have not been evaluated.

Influenza A viruses are subdivided into 16 H (H1-H16) and 9 N (N1-N9) subtypes. Nomenclature for these virus isolates indicate the influenza virus type (A or B), host species (omitted if human), geographical site, year of isolation, and the H and N subtype (example: A/California/14/2009 H1N1). The main antigenic determinants of Influenza A and B viruses are the hemagglutinin (HA) and neuraminidase (NA) transmembrane glycoproteins. NA forms a tetramer with an average size of 220kDa (~55kDa per monomer). The matrix (M) protein of Influenza A virus is one of the two group-specific internal proteins of the virus. The non-structural protein (NP) exists as a homeodimer (~52kDa) consisting of two identical monomers (each ~26kDa).