Indole-3-Butyric Acid (IBA)
Indole-3-Butyric Acid (IBA) Powder is a plant growth regulator widely used to promote root growth in plant tissue culture applications.
Product Specifications
CAS Number
133-32-4
Synonyms
IBA; 4-[3-Indolyl]butyric Acid
HS Code
3808.93
Applications
Stimulates adventitious root development and callus formation.
IBA can be included in media formulations for micropropagation to enhance plantlet root development.
IBA can be applied to the graft union to promote faster callus formation and successful graft healing.
IBA can promote germination in certain seeds with dormancy requirements.
Assay Protocol
Follow the given steps to prepare the tissue culture media using PCT’s IBA powder:
Prepare liquid tissue culture media using PCT’s MS, DKW media, or any other alternative media based on your plant.
Add sugar based on the quantity of media you’re making (generally, 30 grams of sugar is added for 1-liter media), 1 ml/L PPM, and 3-4 grams per liter of gellan gum or 6-8 grams per liter of agar.
Then, add the desired quantity of IBA from the prepared IBA stock solution.
Autoclave the medium for 20 minutes at 15 psi and 121°C.
Your media is ready!
Solubility
Soluble in NaOH or KOH
Molecular Formula
C12H13NO2
Features & Benefits
Easy to use.
Used to supplement tissue culture media, such as MS media.
Stimulates adventitious root development and callus formation.
It is often used with other hormones to promote optimal plant growth and development.
Storage Temperature
2-8°C
Shelf Life
3 years from the manufacturing date
Appearance
Cream to Tan Powder
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