Mouse IgG2a Fc Protein, Tag Free (MALS verified)
Mouse IgG2a Fc, Tag Free (IGA-M5207) is expressed from human 293 cells (HEK293) . It contains AA Glu 98 - Lys 330 (Accession # CAC20702) .
Product Specifications
Background
Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F (ab) s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
Specifications
This protein carries no "tag". The protein has a calculated MW of 26.4 kDa. The protein migrates as 30-35 kDa under reducing (R) condition, and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Accession Number
CAC20702
Expression Region
Glu 98 - Lys 330
Host
HEK293
Target
IgG2a Fc
Conjugation
Unconjugated
Tag
Native
Source
Mouse
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Format
Powder
Buffer
50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
Additives
Trehalose
Molecular Weight
26.4 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
200ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/IGA-M5207.pdf
Species
Mouse
Curated Selection
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