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Mouse IgG2a Fc Protein, Tag Free (MALS verified)

Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F (ab) s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.

Product Specifications

Background

Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F (ab) s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.

Specifications

This protein carries no "tag". The protein has a calculated MW of 26.4 kDa. The protein migrates as 30-35 kDa under reducing (R) condition, and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

CAC20702

Expression Region

Glu 98 - Lys 330

Host

HEK293

Target

IgG2a Fc

Conjugation

Unconjugated

Tag

Native

Source

Mouse

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Format

Powder

Buffer

50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5

Additives

Trehalose

Molecular Weight

26.4 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

RT

Storage Conditions

-20°C

Package Size

200ug*1

Host or Source

HEK293

Species

Mouse

Protein ID

CAC20702

Preservative

Trehalose

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