4-Methylumbelliferyl β-D-Glucopyranoside

4-Methylumbelliferyl β-D-Glucopyranoside, a β-D-glucoside, is a fluorogenic substrate for β-glucosidase, utilizes to assay β-glucosidase activity[1]. 4-Methylumbelliferyl β-D-Glucopyranoside releases the highly fluorescent 4-methylumbelliferyl (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively[2].

Product Specifications

CAS Number

[18997-57-4]

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

Glycosidase

Type

Reference compound

Related Pathways

Metabolic Enzyme/Protease

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/4-methylumbelliferyl-β-d-glucopyranoside.html

Purity

99.87

Solubility

DMF : 50 mg/mL (ultrasonic) |DMSO : 100 mg/mL (ultrasonic)

Smiles

OC[C@@H]1[C@H]([C@@H]([C@H]([C@@H](O1)OC2=CC=C3C(C)=CC(OC3=C2)=O)O)O)O

Molecular Formula

C16H18O8

Molecular Weight

338.31

Precautions

H302, H315, H319, H335

References & Citations

[1]Smitka CM, et al. Rapid fluorogenic assay for differentiation of the Candida parapsilosis group from other Candida spp. J Clin Microbiol. 1989;27 (1) :203-206.|[2]Oftedal L, et al. Validation and assessment of preanalytical factors of a fluorometric in vitro assay for glucocerebrosidase activity in human cerebrospinal fluid. Sci Rep. 2020;10 (1) :22098. Published 2020 Dec 16.|[3]Boado RJ, et al. Bi-functional IgG-lysosomal enzyme fusion proteins for brain drug delivery. Sci Rep. 2019 Dec 9;9 (1) :18632.