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DirectPCR Lysis Reagent (Ear)

Selected Variant

402-E

Size: 50 mL

<font size="3">DirectPCR Lysis Reagent (Ear) is a molecular biology reagent for rapid DNA preparation from mouse ear tissue for direct PCR and genotyping. The single-tube DirectPCR workflow enables preparation of PCR-compatible crude DNA lysates while reducing conventional DNA extraction and transfer steps.</font><div><font size="3"><br></font></div><div><font color="#0115ad" size="5"><b style="">What is </b><b>DirectPCR Lysis Reagent (Ear) | Mouse DNA PCR Genotyping</b></font><span style="color: rgb(1, 21, 173); font-size: x-large;"><b>?</b></span></div><div><font size="3">DirectPCR Lysis Reagent (Ear) is designed for preparation of DNA from mouse ear pieces prior to PCR-based genotyping. The reagent combines tissue lysis with a formulation intended to reduce the inhibitory effects of crude biological lysates on PCR, allowing the resulting lysate to be used as a DNA template in compatible PCR workflows.</font></div><div><font size="5" color="#0115ad"><b style=""><i style=""><br></i></b></font></div><div><font size="5" color="#0115ad"><b style="">Key Features </b></font><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);" color="#0115ad">=&gt;</font><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"> </font></span><font size="3">Designed for mouse ear tissue and ear-punch samples. </font><font color="#0115ad" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: 700;">=&gt;</font><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: 700;"> </font><font size="3">Suitable for direct PCR and genotyping. </font><font color="#0115ad" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: 700;">=&gt;</font><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: 700;"> </font><font size="3">Single-tube DNA preparation workflow </font><font color="#0115ad" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: 700;">=&gt;</font><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: 700;"> </font><font size="3">Reduces sample transfer and DNA purification steps. </font><font color="#0115ad" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: 700;">=&gt;</font><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: 700;"> </font><font size="3">Designed to produce PCR-compatible crude DNA lysates. </font><font color="#0115ad" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: 700;">=&gt;</font><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: 700;"> </font><font size="3">No organic extraction reagents required. </font><font color="#0115ad" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: 700;">=&gt;</font><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: 700;"> </font><font size="3">50 mL format for approximately 500 mouse ear pieces. The manufacturer's current specification states 50 mL / 500 ear pieces for <b>0388-402-E</b>.</font></div>

Product Specifications

Applications

<font size="3"><b><font color="#0a1394">PCR Genotyping =&gt; </font></b>Preparation of DNA templates from mouse ear samples for genomic PCR genotyping. <b><font color="#0a1394">Mouse Genetic Screening </font></b><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=&gt; </font></span>Useful for screening mouse colonies and identifying genotypes from ear-punch tissue. <b><font color="#0a1394">Molecular Biology Research </font></b><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=&gt; </font></span>Suitable for research workflows requiring rapid DNA preparation from animal tissue. There is also real published-use evidence: research papers have used DirectPCR Ear reagent to lyse mouse ear tissue before PCR-based genotyping. <b><font color="#02146e">PubMed Central PMCID :</font> <u>PMC11159904</u></b></font>

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FAQ

<h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer"><font size="3"><b><font color="#0a1394">What is DirectPCR® Lysis Reagent (Ear)? </font></b><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=&gt; </font></span>DirectPCR® Lysis Reagent (Ear) is a PCR lysis reagent designed to prepare DNA from mouse ear tissue for direct PCR and genotyping. <font color="#0a1394"><b>What samples can be used with DirectPCR Ear reagent? </b></font></font></h3><h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer"><font size="3"><span style="color: rgb(10, 19, 148); font-weight: bolder;">=&gt; </span>The reagent is designed for mouse ear pieces and ear-punch tissue. <b><font color="#0a1394">Can DirectPCR Ear reagent be used for PCR genotyping? </font></b></font></h3><h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer"><font size="3"><span style="color: rgb(10, 19, 148); font-weight: bolder;">=&gt; </span>Yes. The resulting crude DNA lysate is designed for use as a template in compatible genomic PCR genotyping workflows. <b><font color="#0a1394">Does DirectPCR Ear include Proteinase K? </font></b></font></h3><h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer"><font size="3"><span style="color: rgb(10, 19, 148); font-weight: bolder;">=&gt; </span>No. Proteinase K is required for the manufacturer's ear-tissue protocol but is not included with the lysis reagent. <b><font color="#0a1394">What is the capacity of the 50 mL format? </font></b></font></h3><h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer"><font size="3"><span style="color: rgb(10, 19, 148); font-weight: bolder;">=&gt; </span>The <b>0388-402-E</b> 50 mL format is specified for approximately 500 mouse ear pieces.</font></h3>

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