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DirectPCR Lysis Reagent (Ear)

Selected Variant

401-E

Size: 25 mL

<font size="3">DirectPCR Lysis Reagent (Ear) is a molecular biology reagent for rapid DNA preparation from mouse ear tissue for direct PCR and genotyping. The single-tube DirectPCR® workflow enables preparation of PCR-compatible crude DNA lysates while reducing conventional DNA extraction and sample transfer steps. </font> <font size="5"><font color="#0a1394"><b>What is DirectPCR Lysis Reagent (Ear) | Mouse DNA PCR Genotyping?</b></font> </font><font size="3">DirectPCR Lysis Reagent (Ear) is designed for the preparation of DNA from mouse ear pieces and ear-punch samples prior to PCR-based genotyping. The reagent combines tissue lysis with a formulation intended to reduce the inhibitory effects of crude biological lysates on PCR, allowing the resulting lysate to be used as a DNA template in compatible PCR workflows. </font> <font color="#0a1394" size="5"><b>Key Features </b></font><font size="3"><b style=""><font color="#0a1394" style="">→ </font></b>Designed for mouse ear tissue and ear-punch samples. <span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">→ </font></span>Suitable for direct PCR and genotyping. <span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">→ </font></span>Single-tube DNA preparation workflow. <span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">→ </font></span>Reduces sample transfer and DNA purification steps. <span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">→ </font></span>Designed to produce PCR-compatible crude DNA lysates. <span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">→ </font></span>No organic extraction reagents required. <span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">→ </font></span>25 mL format for approximately 250 mouse ear pieces.</font>

Product Specifications

Applications

<ul><li><font size="3"><font color="#0a1394"><b>PCR Genotyping → </b></font>Preparation of DNA templates from mouse ear and ear-punch samples for genomic PCR genotyping. </font></li><li><font size="3"><b><font color="#0a1394">Mouse Genetic Screening →</font></b> Supports screening of genetically engineered mouse colonies and identification of genotypes from ear-punch tissue. </font></li><li><font size="3"><b><font color="#0a1394">Molecular Biology Research → </font></b>Suitable for research workflows requiring rapid preparation of DNA from mouse tissue for downstream PCR analysis.</font></li></ul><div><font size="3"><br></font></div><font size="3"><b><font color="#0a1394">Research Use : </font></b>DirectPCR lysis-based approaches have been used in published research for mouse ear tissue lysis and PCR-based genotyping, supporting the application of rapid crude DNA preparation in mouse genetic screening workflows. </font><br>

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FAQ

<b><font color="#0a1394" size="3">What is DirectPCR Lysis Reagent (Ear)? </font></b><h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><font size="3"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=&gt; </font></span>DirectPCR Lysis Reagent (Ear) is a PCR lysis reagent designed to prepare DNA from mouse ear tissue for direct PCR and genotyping.</font></h3><font size="3"> <b><font color="#0a1394">What samples can be used with DirectPCR Ear Reagent? </font></b></font><h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><font size="3"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=&gt; </font></span>The reagent is designed for mouse ear pieces and ear-punch tissue used in PCR-based genotyping workflows.</font></h3><font size="3"> <b><font color="#0a1394">Can DirectPCR Ear Reagent be used for PCR genotyping? </font></b></font><h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><font size="3"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=&gt; </font></span>Yes. The resulting crude DNA lysate is designed for use as a template in compatible genomic PCR genotyping workflows.</font></h3><font size="3"> <b><font color="#0a1394">Does DirectPCR Ear Reagent include Proteinase K? </font></b></font><h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><font size="3"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=&gt; </font></span>No. Proteinase K is required for the manufacturer's ear-tissue protocol but is not included with the DirectPCR Lysis Reagent.</font></h3><font size="3"> <b><font color="#0a1394">What is the capacity of the 25 mL format? </font></b></font><h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><font size="3"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#0a1394" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=&gt; </font></span>The <b style="">0388-401-E</b> 25 mL format is the smaller DirectPCR Ear reagent configuration. If the manufacturer has not explicitly specified the number of ear pieces for this size, I recommend not stating a numerical capacity on the product page.</font></h3>

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