Sheep anti Human chx 10 (Visual system homeobox 2) (NT)

Chx10 is a 46kDa homeodomain protein of the paired-like class that is essential for development of the mammalian eye. Mutations in Chx10 cause microphthalmia, a cause of congenital blindness in Humans, and the ocular retardation (or) phenotype in mice. In the developing mouse retina Chx10 is expressed in retinal progenitors, while in the mature retina, Chx10 expression becomes restricted to bipolar neurons. Concurrent with these expression patterns, the Chx10-/- (or) retina is thin due to a defect in proliferation of retinal progenitors, and lacks bipolar neurons. Chx10 is also expressed in the developing brainstem, thalamus, and spinal cord.

Antibody raised in sheep against a recombinant protein corresponding to amino acids 1 to 131 derived from the N-terminal region of the Human Chx10 protein conjugated to KLH.

Detects Chx10 by Western blot at 0.5 to 1 µg/mL. Detects a 46 kDa band in mouse and rat retinal tissue lysates. Optimal concentration should be evaluated by serial dilutions. Suitable for use against recombinant proteins conjµgated to OVA, GST, His tags and other.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

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