Caspase 3/7 Activity Detection Substrate for Flow Cytometry

The Caspase 3/7 Substrates (Green) are based on novel fluorogenic DNA dyes that have been coupled to the caspase 3/7 recognition sequence (DEVD), which is both non-fluorescent and nonfunctional as a DNA dye.When it rapidly crosses cell membranes to enter the cytoplasm and cleaved by caspase 3/7 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the Caspase 3/7 substrates allow detection of caspase 3/7 activity and visualization of apoptotic nuclear morphology simultaneously.