Anti-dsRNA monoclonal antibody J5

SCICONS anti-dsRNA monoclonal antibody J5 recognizes double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I).poly(C) and poly(A).poly(U) have been recognised by J5, although in some assays its affinity to poly(I).poly(C) is about 10 times lower than that to other dsRNA antigens.

To prepare a 1 μg/μl PBS antibody solution add 200 μl (RNT-SCI-10040200) or 500 μl (RNT-SCI-10040500) sterile DNAse/RNAse-free distilled Water. As a result of the lyophilisation procedure, the reconstituted antibody may contain small amounts of denatured protein in the form of aggregates that may interfere with some applications such as immunohistochemistry (e.g. by giving high backgrounds). We therefore highly recommend to spin down (microcentrifuge) the reconstituted antibody before use and to use the supernatant only.