Human NODAL Protein, Tag Free

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. This protein is required for maintenance of human embryonic stem cell pluripotency and may play a role in human placental development. Mutations in this gene are associated with heterotaxy, a condition characterized by random orientation of visceral organs with respect to the left-right axis. [provided by RefSeq, Aug 2016]

Product Specifications

Background

Specifications

This protein carries no "tag". The protein has a calculated MW of 12.9 kDa. The protein migrates as 14-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Accession Number

Q96S42

Expression Region

His 238 - Leu 347

Host

E. coli

Target

NODAL

Conjugation

Unconjugated

Tag

Native

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human NODAL Protein, Tag Free (Cat. No. NOL-H5133) at 10 μg/mL (100 μL/well) can bind Human SIRP gamma, Fc Tag (Cat. No. SIG-H5253) with a linear range of 0.01-0.625 μg/mL (QC tested).