B7-2/CD86 Polyclonal Antibody
Product Specifications
Background
B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily.
Abbreviation
CD86
UniProt
O35531
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant Rat B7-2/CD86 protein expressed by Mammalian
Target
Activation B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86; CD28LG2B7-2 antigen) ; CD28 antigen ligand 2
Clonality
Polyclonal
Conjugation
Unconjugated
Applications
WB; IHC
Purification
Antigen Affinity Purification
Concentration
1 mg/mL
Dilution
WB 1:500-1:1000; IHC 1:200-1:400
Buffer
PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol, pH7.4
Shipping Conditions
The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
Storage Conditions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Calculated Molecular Weight
37KDa
Observed Molecular Weight
75kDa
Isotype
IgG
Available Sizes
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