EcoPlexTM Human Inflammation 16-Plex Panel Kit

MIP-1α:P10147 TNF-α:P01375 IP-10:P02778 IL-1β:P01584 IL-27:Q8NEV9 IFN-γ:P01579 IL-8:P10145 IL-31:Q6EBC2 IL-12p70:P29459&P29460 MCP-1:P13500 IL-1α:P01583 IL-6:P05231 IL-33:O95760 IL-17A:Q16552 IL-10:P22301 IL-13:P35225

EcoPlexTM CBA kits utilize antibodies-conjugated beads which are different in size and mean fluorescent intensity of labeling dye to capture antigens in sample. After capture beads and sample incubation, biotin-conjugated detection antibodies are added to react with antigen-beads complexes, followed by a streptavidin-R-phycoerythrin (SA-PE) treatment. The fluorescent intensity of PE on each bead populations is quantified by flow cytometer with either a single 488nm laser or dual 488nm and 633/640nm lasers. The maximum emission of the bead classification dye is at 700nm. The concentrations of protein interested are determined by comparing the fluorescent intensities to those of standard curve, like sandwich ELISA data analysis.

MIP-1α<2pg/mL TNF-α<1pg/mL IP-10<3pg/mL IL-1β<5pg/mL IL-27<5pg/mL IFN-γ<3pg/mL IL-8<1pg/mL IL-31<5pg/mL IL-12p70<3pg/mL MCP-1<5pg/mL IL-1α<2pg/mL IL-6<5pg/mL IL-33<5pg/mL IL-17A<3pg/mL IL-10<2pg/mL IL-13<5pg/mL

This kit is for detection of IFN-γ, IL-1α, IL-1β, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, IL-27, IL-31, IL-33, IP-10, MCP-1, MIP-1α and TNF-α in human serum, plasma, biological fluid and cell culture supernatant.