Anti-IL-1RacP (CT) Antibody

Rabbit Polyclonal Antibody specific to IL-1RAcP (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

IL-1 receptor accessory protein, IL-1RAcP, EC 3.2.2.6, Interleukin-1 receptor 3, IL-1R-3, IL-1R3

Gene Name

IL1RAP

Gene ID

3556

NCBI Gene ID

UniProt

Q9NPH3

Accession Number

NP_001161401

Cellular Locus

[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Isoform 3]: Secreted.

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 549-569 of human IL-1RAcP. The sequence differs from mouse IL-1RacP by three amino acids.

Target Antigen

Interleukin-1 receptor accessory protein

Target

IL-1RacP (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Coreceptor for IL1RL2 in the IL-36 signaling system (By similarity). Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 dUniProtKB:Q61730, PubMed:10799889, PubMed:9371760, PubMed:10653850, PubMed:19836339}.; [Isoform 2]: Associates with secreted ligand-bound IL1R2 and increases the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors (PubMed:12530978). Enhances the ability of secreted IL1R1 to inhibit IL-33 signaling (By similarity). {UniProtKB:Q61730, PubMed:12530978}.; [Isoform 4]: Unable to mediate canonical IL-1 signaling (PubMed:19481478). Required for Src phosphorylation by IL1B. May be involved in IL1B-potentiated NMDA-induced calcium influx in neurons (By similarity). {UniProtKB:Q61730, PubMed:19481478}.

Additionnal Information

Immunoblotting: use at 1:500-1:1,000 dilution. <br><br>Positive control: Whole cell lysate from HeLa cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human IL- 1RAcP (66 kD). No cross-reactivity with other members of the IL-1 receptor family.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Cellular responses induced by the pro- inflammatory cytokine IL-1 require two subunits of the IL-1 receptor, IL-1 receptor 1 (IL-1R1) and IL-1 receptor accessory protein (IL-1RacP). IL- 1RacP forms a complex with IL-1R1 in response to IL-1 treatment. IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kappaB inducing kinase (NIK) and NF-kappaB and associates with the IL-1Rcomplex through IL- 1RacP. IL-1 activation of stress- activated protein kinase andof acid sphingomyelinase also requires IL- 1RacP. Like IL-1R1, the IL-1RacP subunit is essential for IL-1 mediated cellular responses. IL-1RacP is expressed in a variety of tissues.