HSV-1 (strain 17) Envelope Glycoprotein D (gD), His Tag (MALS verified)

HSV-1 (strain 17) Envelope Glycoprotein D (gD), His Tag (GLD-V52H3) is expressed from human 293 cells (HEK293) . It contains AA Lys 26 - Thr 310 (Accession # Q69091-1) .

Product Specifications

Background

Herpesvirus infections are widely spread throughout the world population. Herpes simplex virus (HSV) belongs to the α-herpesvirus subfamily. There are two main types of HSV, HSV-1 and HSV-2, which infect humans. HSV-2 mainly causes genital lesions, whereas HSV-1 is involved in both oral and genital infections. Glycoprotein D (gD) is a structural component of the herpes simplex virus type 1 (HSV-1) envelope which is essential for virus entry and fusion with host cells. gD plays an important role by binding to the host receptors such as herpes virus entry mediator (HVEM) and nectin-1, a member of the immunoglobulin (Ig) -like cell adhesion molecules.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.5 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q69091-1

Expression Region

Lys 26 - Thr 310

Host

HEK293

Target

Glycoprotein D/gD (HSV)

Conjugation

Unconjugated

Tag

C-10xHis

Source

HSV-1

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized HSV-1 (strain 17) Envelope Glycoprotein D (gD), His Tag (Cat. No. GLD-V52H3) at 5 μg/mL (100 μL/well) can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with a linear range of 0.3-5 ng/mL (QC tested).