EXTRAClean Urine Exosome and Free-Circulating RNA Isolation Maxi Kit

Versatile sample input ranges. The purified exosomal RNA is free from any circulating RNA-binding proteins. No phenol extractions, Proteinase K treatment nor carrier RNA required. No time-consuming ultracentrifugation, filtration nor special syringes are required. No precipitation reagents nor overnight incubation required. Concentrate isolated exosomal RNA and free-circulating RNA into a flexible elution volume ranging from 50 μL to 100 μL. Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix. Norgen’s EXTRAClean Urine Exosome and Free-Circulating RNA Isolation Kit constitutes an all-in-one system for the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from different urine sample volumes ranging from 250 μL to 30 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, NGS application, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.