Mycoplasma TaqMan PCR Kit

Detection kits for Mycoplasma DNA Available in TaqMan format for analysis Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, M. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines. Mycoplasma TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Mycoplasma TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics.