Candidatus Liberibacter TaqMan Probe/Primer and Control Set

Detection kits for the Candidatus Liberibacter Available in TaqMan format for analysis Citrus Huanglongbing (HLB), previously known as citrus greening, constitutes the most economically devastating disease of citrus worldwide, being present in around 40 countries. HLB causes severe symptoms, depending on the age and the number of infections per plant. The disease is caused by the fastidious gram-negative α-proteobacteria Candidatus Liberibacter asiaticus (Las), Ca. L. africanus, and Ca. L. americanus as well as some phytoplasmas. The bacterium propagates within the phloem of citrus plants producing die-back, yellow shoots, blotchy mottles on leaves, and off-tasting and malformed fruit. The ‘Ca. Liberibacter’ bacterium is transmitted by the psyllids Trioza erytreae in Africa and Diaphorina citri in Asia and the Americas. A variety of diagnostic methods have been developed to supplement or confirm field-based diagnosis of HLB however, end-point PCR-based and real-time quantitative methods are currently the most widely used methods in plant and insect tissues to detect and identify HLB- Liberibacters. Candidatus Liberibacter TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Candidatus Liberibacter TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics.