AAV Quantification Kit

Rapid AAV DNA extraction and quantification can be completed in 2 hours Universal qPCR assay targeting the AAV2 ITR enables quantification of most AAV vector constructs Purified DNA standard enables easy comparison of data between different lab groups Non-plasmid based standard prevents quantification artifacts due to insufficient melting of plasmid sequences DNAse digestion step aids in eliminating non-viral DNA Minimal sample handling for maximum AAV DNA recovery Norgen’s AAV Quantification Kit is designed for the detection of adeno-associated virus (AAV) inverted terminal repeat (ITR) sequences in a real-time PCR based on the use of TaqMan® technology. A purified standard based solely on the AAV2 ITR sequence purified using Norgen’s proprietary silicon carbide technology simplifies the generation of a reliable standard curve for AAV quantification. Avoidance of a plasmid based standard eliminates problems associated with efficient melting of the ITR sequence due to coupling of the ITR to the much longer plasmid sequence, as well as variability due to rearrangements/duplications/deletions of the recombination prone ITR. An easy and rapid method for viral DNA extraction simplifies the step of obtaining AAV DNA while simultaneously eliminating contaminating non-encapsidated DNA. Norgen’s AAV Quantification Kit can facilitate pre-clinical studies that require accurate vector titration as well as interlab comparisons of vector quantities. This kit is designed for research use only and not for use in diagnostic procedures.