Urine Exosome Purification and RNA Isolation Midi Kit

Purification and enrichment of intact urinary exosomes for functional studies Bind and elute all RNA irrespective of size or GC content, without bias Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. Versatile urine input volumes No phenol extractions, Proteinase K treatment, nor carrier RNA required No time-consuming ultracentrifugation, filtration nor special syringes are required No precipitation reagents nor overnight incubation required Compatible with urine from any species Pure exosomes are purified and are free-from any other RNA-binding proteins Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services Norgen’s Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of exosomal RNA from different urine sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Urine Exosome Purification and RNA Isolation Mini Kit For sample volumes ranging from 250 µL to 1 mL. Urine Exosome Purification and RNA Isolation Midi Kit For sample volumes ranging from 2 mL to 10 mL. Urine Exosome Purification and RNA Isolation Maxi Kit For sample volumes ranging from 11 mL to 30 mL.