Cell Culture Media Exosome Purification and RNA Isolation Mini Kit

Purification and enrichment of intact cell culture media exosomes for functional studies Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. Bind and elute all RNA irrespective of size or GC content, without bias Versatile cell culture media input volumes No phenol extractions, Proteinase K treatment, nor carrier RNA required No time-consuming ultracentrifugation, filtration nor special syringes required No precipitation reagents nor overnight incubation required Pure exosomes are purified and are free-from any other RNA-binding proteins Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen's Cell Culture Media Exosome Purification and RNA Isolation Kits constitute an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any RNA-binding proteins and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Cell Culture Media Exosome Purification and RNA Isolation Mini Kit For sample input volumes ranging from 5 mL to 10 mL. Cell Culture Media Exosome Purification and RNA Isolation Midi Kit For sample input volumes ranging from 10 mL to 20 mL. Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit For sample input volumes ranging from 20 mL to 35 mL.