Urine Exosome Purification Midi Kit

Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes. Versatile sample input ranging from 250 µL to 30 mL Urine Exosome Purification Mini Kit (250 µL - 1 mL urine) Urine Exosome Purification Midi Kit (2 mL - 10 mL urine) Urine Exosome Purification Maxi Kit (11 mL - 30 mL urine) Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins. No precipitation reagents, overnight incubation, protease or coagulant treatments required No time-consuming ultracentrifugation, filtration or special syringes required Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type Purified exosomes are compatible with functional studies. Purified exosomes are free from any protein-bound cell-free circulating RNA Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration. Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method. The Urine Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different urine sample volumes ranging from 250 µL to 30 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different urine sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression. NanoSight® Analysis Exosomes enriched with Norgen’s Urine Exosome Purification Kits can be analyzed using NanoSight® for assessing the approximate exosome size range and concentration. Exosomal RNA Analysis Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Urine Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery.